TY  - JOUR
A1  - Naseri, Gita
A1  - Balazadeh, Salma
A1  - Machens, Fabian
A1  - Kamranfar, Iman
A1  - Messerschmidt, Katrin
A1  - Müller-Röber, Bernd
T1  - Plant-Derived Transcription Factors for Orthologous Regulation of Gene Expression in the Yeast Saccharomyces cerevisiae
T2  - ACS synthetic biology
N2  - Control of gene expression by transcription factors (TFs) is central in many synthetic biology projects for which a tailored expression of one or multiple genes is often needed. As TFs from evolutionary distant organisms are unlikely to affect gene expression in a host of choice, they represent excellent candidates for establishing orthogonal control systems. To establish orthogonal regulators for use in yeast (Saccharomyces cerevisiae), we chose TFs from the plant Arabidopsis thaliana. We established a library of 106 different combinations of chromosomally integrated TFs, activation domains (yeast GAL4 AD, herpes simplex virus VP64, and plant EDLL) and synthetic promoters harboring cognate cis regulatory motifs driving a yEGFP reporter. Transcriptional output of the different driver/reporter combinations varied over a wide spectrum, with EDLL being a considerably stronger transcription activation domain in yeast than the GAL4 activation domain, in particular when fused to Arabidopsis NAC TFs. Notably, the strength of several NAC-EDLL fusions exceeded that of the strong yeast TDH3 promoter by 6- to 10-fold. We furthermore show that plant TFs can be used to build regulatory systems encoded by centromeric or episomal plasmids. Our library of TF-DNA binding site combinations offers an excellent tool for diverse synthetic biology applications in yeast.
KW  - Arabidopsis thaliana
KW  - artificial transcription factor
KW  - NAC transcription factor
KW  - synthetic biology
KW  - plant
Y1  - 2017
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/46383
SN  - 2161-5063
VL  - 6
SP  - 1742
EP  - 1756
PB  - American Chemical Society
CY  - Washington
ER  -