TY  - GEN
A1  - Zhang, Youjun
A1  - Chen, Moxian
A1  - Siemiatkowska, Beata
A1  - Toleco, Mitchell Rey
A1  - Jing, Yue
A1  - Strotmann, Vivien
A1  - Zhang, Jianghua
A1  - Stahl, Yvonne
A1  - Fernie, Alisdair R.
T1  - A highly efficient agrobacterium-mediated method for transient gene expression and functional studies in multiple plant species
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Although the use of stable transformation technology has led to great insight into gene function, its application in high-throughput studies remains arduous. Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis, but this technique does not work efficiently in other plant species, including Arabidopsis thaliana. As an efficient high-throughput transient expression system is currently lacking in the model plant species A. thaliana, we developed a method that is characterized by high efficiency, reproducibility, and suitability for transient expression of a variety of functional proteins in A. thaliana and 7 other plant species, including Brassica oleracea, Capsella rubella, Thellungiella salsuginea, Thellungiella halophila, Solanum tuberosum, Capsicum annuum, and N. benthamiana. Efficiency of this method was independently verified in three independent research facilities, pointing to the robustness of this technique. Furthermore, in addition to demonstrating the utility of this technique in a range of species, we also present a case study employing this method to assess protein-protein interactions in the sucrose biosynthesis pathway in Arabidopsis.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1189 
KW  - transient expression
KW  - agro-infiltration
KW  - subcellular localization
KW  - protein-protein interaction
Y1  - 2020
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/52425
UR  - https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-524254
SN  - 1866-8372
IS  - 5
ER  -