TY  - GEN
A1  - Bálint, Miklós
A1  - Márton, Orsolya
A1  - Schatz, Marlene
A1  - Düring, Rolf-Alexander
A1  - Grossart, Hans-Peter
T1  - Proper experimental design requires randomization/balancing of molecular ecology experiments
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laboratory events. We demonstrate this with a simple experiment where unexpected differences in laboratory processing of samples would have biased results if randomization in DNA extraction and PCR steps do not provide safeguards. We emphasize the need for proper experimental design and reporting of the laboratory phase of molecular ecology research to ensure the reliability and interpretability of results.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 616 
KW  - batch effect
KW  - bias
KW  - DNA extraction
KW  - environmental DNA
KW  - laboratory practice
KW  - lake community
KW  - metabarcoding
KW  - nondemonic intrusions
KW  - PCR
KW  - sediment
Y1  - 2019
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/42387
UR  - https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-423878
SN  - 1866-8372
IS  - 616
ER  -