TY  - JOUR
A1  - Kalimuthu, Palraj
A1  - Leimkühler, Silke
A1  - Bernhardt, Paul V.
T1  - Xanthine dehydrogenase electrocatalysis autocatalysis and novel activity
T2  - The journal of physical chemistry : B, Condensed matter, materials, surfaces, interfaces & biophysical chemistry
N2  - The enzyme xanthine dehydrogenase (XDH) from the purple photosynthetic bacterium Rhodobacter capsulatus catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid as part of purine metabolism. The native electron acceptor is NAD(+) but herein we show that uric acid in its 2-electron oxidized form is able to act as an artificial electron acceptor from XDH in an electrochemically driven catalytic system. Hypoxanthine oxidation is also observed with the novel production of uric acid in a series of two consecutive 2-electron oxidation reactions via xanthine. XDH exhibits native activity in terms of its pH optimum and inhibition by allopurinol.
Y1  - 2011
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/36952
SN  - 1520-6106
VL  - 115
IS  - 11
SP  - 2655
EP  - 2662
PB  - American Chemical Society
CY  - Washington
ER  -