TY  - JOUR
A1  - Caserta, Giorgio
A1  - Zhang, Xiaorong
A1  - Yarman, Aysu
A1  - Supala, Eszter
A1  - Wollenberger, Ulla
A1  - Gyurcsányi, Róbert  E.
A1  - Zebger, Ingo
A1  - Scheller, Frieder W.
T1  - Insights in electrosynthesis, target binding, and stability of peptide-imprinted polymer nanofilms
T2  - Electrochimica acta : the journal of the International Society of Electrochemistry (ISE)
N2  - Molecularly imprinted polymer (MIP) nanofilms have been successfully implemented for the recognition of different target molecules: however, the underlying mechanistic details remained vague. 
This paper provides new insights in the preparation and binding mechanism of electrosynthesized peptide-imprinted polymer nanofilms for selective recognition of the terminal pentapeptides of the beta-chains of human adult hemoglobin, HbA, and its glycated form HbA1c. 
To differentiate between peptides differing solely in a glucose adduct MIP nanofilms were prepared by a two-step hierarchical electrosynthesis that involves first the chemisorption of a cysteinyl derivative of the pentapeptide followed by electropolymerization of scopoletin. 
This approach was compared with a random single-step electrosynthesis using scopo-letin/pentapeptide mixtures. Electrochemical monitoring of the peptide binding to the MIP nanofilms by means of redox probe gating revealed a superior affinity of the hierarchical approach with a Kd value of 64.6 nM towards the related target. 
Changes in the electrosynthesized non-imprinted polymer and MIP nanofilms during chemical, electrochemical template removal and rebinding were substantiated in situ by monitoring the characteristic bands of both target peptides and polymer with surface enhanced infrared absorption spectroscopy. 
This rational approach led to MIPs with excellent selectivity and provided key mechanistic insights with respect to electrosynthesis, rebinding and stability of the formed MIPs.
KW  - SEIRA spectroelectrochemistry
KW  - peptide imprinting
KW  - electrosynthesis
KW  - MIP
KW  - glycated peptide
Y1  - 2021
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/62989
SN  - 0013-4686
SN  - 1873-3859
VL  - 381
PB  - Elsevier
CY  - New York, NY [u.a.]
ER  -