TY  - GEN
A1  - Yang, Lei
A1  - Tang, Renjie
A1  - Zhu, Jinqi
A1  - Liu, Hua
A1  - Mueller-Roeber, Bernd
A1  - Xia, Huijun
A1  - Zhang, Hongxia
T1  - Enhancement of stress tolerance in transgenic tobacco plants constitutively expressing AtIpk2β, an inositol polyphosphate 6-/3-kinase from Arabidopsis thaliana
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Inositol phosphates (IPs) and their turnover products have been implicated to play important roles in stress signaling in eukaryotic cells. In higher plants genes encoding inositol polyphosphate kinases have been identified previously, but their physiological functions have not been fully resolved. Here we expressed Arabidopsis inositol polyphosphate 6-/3-kinase (AtIpk2 beta) in two heterologous systems, i.e. the yeast Saccharomyces cerevisiae and in tobacco (Nicotiana tabacum), and tested the effect on abiotic stress tolerance. Expression of AtIpk2 beta rescued the salt-, osmotic- and temperature-sensitive growth defects of a yeast mutant strain (arg82 Delta) that lacks inositol polyphosphate multikinase activity encoded by the ARG82/IPK2 gene. Transgenic tobacco plants constitutively expressing AtIpk2 beta under the control of the Cauliflower Mosaic Virus 35S promoter were generated and found to exhibit improved tolerance to diverse abiotic stresses when compared to wild type plants. Expression patterns of various stress responsive genes were enhanced, and the activities of anti-oxidative enzymes were elevated in transgenic plants, suggesting a possible involvement of AtIpk2 beta in plant stress responses.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 954 
KW  - arabidopsis thaliana
KW  - AtIpk2 beta
KW  - inositol phosphate
KW  - IP3
KW  - stress tolerance
KW  - transgenic tobacco
Y1  - 2020
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/43122
UR  - https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-431225
SN  - 1866-8372
IS  - 954
ER  -