TY  - JOUR
A1  - Hassan, Rabeay Y. A.
A1  - Wollenberger, Ulla
T1  - Direct determination of bacterial cell viability using carbon nanotubes modified screen-printed electrodes
T2  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
N2  - For the early detection of bacterial infection, there is a need for rapid, sensitive, and label-free assays. Thus, in this study, nanostrucured microbial electrochemical platform is designed to monitor the viability and cell growth of S. aureus. Using multi-walled carbon nanotube modified screen-printed electrodes (MWCNTs/SPE), the cyclic voltammetric measurements showed only one irreversible oxidation peak at 600 mV vs Ag/AgCl that accounts for the viable and metabolically active bacterial cells. The assay was optimized and the secreted metabolites, in the extracellular matrix, were directly detected. The peak current showed a positive correlation with viable cell numbers ranging from OD600 nm of 0.1 to 1.1, indicating that the activity of live cells can be quantified. Consequently, responses of viable and non-viable cells of S. aureus to the effects of antibiotic and respiratory chain inhibitors were determined. Thus, the proposed nanostructure-based bacterial sensor provides a reasonable and reliable way for real-time monitoring of live-dead cell functions, and antibacterial profiling.
KW  - Bacterial biosensor
KW  - cell viability assay
KW  - screen printed electrodes (SPEs)
KW  - carbon nanotubes (CNTs)
Y1  - 2019
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/49127
SN  - 1040-0397
SN  - 1521-4109
VL  - 31
IS  - 6
SP  - 1112
EP  - 1117
PB  - Wiley-VCH
CY  - Weinheim
ER  -