TY - JOUR A1 - Nowak, Jacqueline A1 - Gennermann, Kristin A1 - Persson, Staffan A1 - Nikoloski, Zoran T1 - CytoSeg 2.0 T2 - Bioinformatics N2 - Motivation: Actin filaments (AFs) are dynamic structures that substantially change their organization over time. The dynamic behavior and the relatively low signal-to-noise ratio during live-cell imaging have rendered the quantification of the actin organization a difficult task. Results: We developed an automated image-based framework that extracts AFs from fluorescence microscopy images and represents them as networks, which are automatically analyzed to identify and compare biologically relevant features. Although the source code is freely available, we have now implemented the framework into a graphical user interface that can be installed as a Fiji plugin, thus enabling easy access by the research community. Y1 - 2020 UR - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/59907 SN - 1367-4803 SN - 1460-2059 VL - 36 IS - 9 SP - 2950 EP - 2951 PB - Oxford Univ. Press CY - Oxford ER -