TY  - JOUR
A1  - Dortay, Hakan
A1  - Müller-Röber, Bernd
T1  - A highly efficient pipeline for protein expression in Leishmania tarentolae using infrared fluorescence protein as marker
N2  - Background: Leishmania tarentolae, a unicellular eukaryotic protozoan, has been established as a novel host for recombinant protein production in recent years. Current protocols for protein expression in Leishmania are, however, time consuming and require extensive lab work in order to identify well-expressing cell lines. Here we established an alternative protein expression work-flow that employs recently engineered infrared fluorescence protein (IFP) as a suitable and easy-to-handle reporter protein for recombinant protein expression in Leishmania. As model proteins we tested three proteins from the plant Arabidopsis thaliana, including a NAC and a type-B ARR transcription factor. Results: IFP and IFP fusion proteins were expressed in Leishmania and rapidly detected in cells by deconvolution microscopy and in culture by infrared imaging of 96-well microtiter plates using small cell culture volumes (2 mu L
Y1  - 2010
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/32135
UR  - http://www.microbialcellfactories.com/home/
SN  - 1475-2859
ER  -