TY  - JOUR
A1  - Zhang, Gong
A1  - Hubalewska, Magdalena
A1  - Ignatova, Zoya
T1  - Transient ribosomal attenuation coordinates protein synthesis and co-translational folding
N2  - Clustered codons that pair to low-abundance tRNA isoacceptors can form slow-translating regions in the mRNA and cause transient ribosomal arrest. We report that folding efficiency of the Escherichia coli multidomain protein Sufl can be severely perturbed by alterations in ribosome-mediated translational attenuation. Such alterations were achieved by global acceleration of the translation rate with tRNA excess in vitro or by synonymous substitutions to codons with highly abundant tRNAs both in vitro and in vivo. Conversely, the global slow-down of the translation rate modulated by low temperature suppresses the deleterious effect of the altered translational attenuation pattern. We propose that local discontinuous translation temporally separates the translation of segments of the peptide chain and actively coordinates their co-translational folding.
Y1  - 2009
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/32019
UR  - http://www.nature.com/nsmb/
SN  - 1545-9985
ER  -