TY  - JOUR
A1  - Wurzbacher, Christian
A1  - Attermeyer, Katrin
A1  - Kettner, Marie Therese
A1  - Flintrop, Clara
A1  - Warthmann, Norman
A1  - Hilt, Sabine
A1  - Grossart, Hans-Peter
A1  - Monaghan, Michael T.
T1  - DNA metabarcoding of unfractionated water samples relates phyto-, zoo- and bacterioplankton dynamics and reveals a single-taxon bacterial bloom
T2  - Environmental microbiology reports
N2  - Most studies of aquatic plankton focus on either macroscopic or microbial communities, and on either eukaryotes or prokaryotes. This separation is primarily for methodological reasons, but can overlook potential interactions among groups. Here we tested whether DNA metabarcoding of unfractionated water samples with universal primers could be used to qualitatively and quantitatively study the temporal dynamics of the total plankton community in a shallow temperate lake. Significant changes in the relative proportions of normalized sequence reads of eukaryotic and prokaryotic plankton communities over a 3-month period in spring were found. Patterns followed the same trend as plankton estimates measured using traditional microscopic methods. The bloom of a conditionally rare bacterial taxon belonging to Arcicella was characterized, which rapidly came to dominate the whole lake ecosystem and would have remained unnoticed without metabarcoding. The data demonstrate the potential of universal DNA metabarcoding applied to unfractionated samples for providing a more holistic view of plankton communities.
Y1  - 2017
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/46488
SN  - 1758-2229
VL  - 9
SP  - 383
EP  - 388
PB  - Wiley
CY  - Hoboken
ER  -