TY  - GEN
A1  - Barlow, Axel
A1  - Hartmann, Stefanie
A1  - Gonzalez, Javier
A1  - Hofreiter, Michael
A1  - Paijmans, Johanna L. A.
T1  - Consensify
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - A standard practise in palaeogenome analysis is the conversion of mapped short read data into pseudohaploid sequences, frequently by selecting a single high-quality nucleotide at random from the stack of mapped reads. This controls for biases due to differential sequencing coverage, but it does not control for differential rates and types of sequencing error, which are frequently large and variable in datasets obtained from ancient samples. These errors have the potential to distort phylogenetic and population clustering analyses, and to mislead tests of admixture using D statistics. We introduce Consensify, a method for generating pseudohaploid sequences, which controls for biases resulting from differential sequencing coverage while greatly reducing error rates. The error correction is derived directly from the data itself, without the requirement for additional genomic resources or simplifying assumptions such as contemporaneous sampling. For phylogenetic and population clustering analysis, we find that Consensify is less affected by artefacts than methods based on single read sampling. For D statistics, Consensify is more resistant to false positives and appears to be less affected by biases resulting from different laboratory protocols than other frequently used methods. Although Consensify is developed with palaeogenomic data in mind, it is applicable for any low to medium coverage short read datasets. We predict that Consensify will be a useful tool for future studies of palaeogenomes.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1033 
KW  - palaeogenomics
KW  - ancient DNA
KW  - sequencing error
KW  - error reduction
KW  - D statistics
KW  - bioinformatics
Y1  - 2020
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/47252
UR  - https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-472521
SN  - 1866-8372
IS  - 1033
ER  -