TY  - JOUR
A1  - Tikhonenko, Irina
A1  - Magidson, Valentin
A1  - Gräf, Ralph
A1  - Khodjakov, Alexey
A1  - Koonce, Michael P.
T1  - A kinesin-mediated mechanism that couples centrosomes to nuclei
T2  - Cellular and molecular life sciences
N2  - The M-type kinesin isoform, Kif9, has recently been implicated in maintaining a physical connection between the centrosome and nucleus in Dictyostelium discoideum. However, the mechanism by which Kif9 functions to link these two organelles remains obscure. Here we demonstrate that the Kif9 protein is localized to the nuclear envelope and is concentrated in the region underlying the centrosome point of attachment. Nuclear anchorage appears mediated through a specialized transmembrane domain located in the carboxyl terminus. Kif9 interacts with microtubules in in vitro binding assays and effects an endwise depolymerization of the polymer. These results suggest a model whereby Kif9 is anchored to the nucleus and generates a pulling force that reels the centrosome up against the nucleus. This is a novel activity for a kinesin motor, one important for progression of cells into mitosis and to ensure centrosome-nuclear parity in a multinuclear environment.
KW  - Centrosome
KW  - Kinesin
KW  - Microtubule
KW  - Dictyostelium
Y1  - 2013
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/35125
SN  - 1420-682X
VL  - 70
IS  - 7
SP  - 1285
EP  - 1296
PB  - Springer
CY  - Basel
ER  -