TY  - JOUR
A1  - Schulze, Sven
A1  - Wehrhold, Michel
A1  - Hille, Carsten
T1  - Femtosecond-Pulsed laser written and etched fiber bragg gratings for fiber-optical biosensing
T2  - Sensors
N2  - We present the development of a label-free, highly sensitive fiber-optical biosensor for online detection and quantification of biomolecules. Here, the advantages of etched fiber Bragg gratings (eFBG) were used, since they induce a narrowband Bragg wavelength peak in the reflection operation mode. The gratings were fabricated point-by-point via a nonlinear absorption process of a highly focused femtosecond-pulsed laser, without the need of prior coating removal or specific fiber doping. The sensitivity of the Bragg wavelength peak to the surrounding refractive index (SRI), as needed for biochemical sensing, was realized by fiber cladding removal using hydrofluoric acid etching. For evaluation of biosensing capabilities, eFBG fibers were biofunctionalized with a single-stranded DNA aptamer specific for binding the C-reactive protein (CRP). Thus, the CRP-sensitive eFBG fiber-optical biosensor showed a very low limit of detection of 0.82 pg/L, with a dynamic range of CRP detection from approximately 0.8 pg/L to 1.2 mu g/L. The biosensor showed a high specificity to CRP even in the presence of interfering substances. These results suggest that the proposed biosensor is capable for quantification of CRP from trace amounts of clinical samples. In addition, the adaption of this eFBG fiber-optical biosensor for detection of other relevant analytes can be easily realized.
KW  - fiber Bragg gratings
KW  - ultra-fast laser inscription
KW  - fiber etching
KW  - nanostructure fabrication
KW  - fiber-optical sensors
KW  - aptamers
KW  - C-reactive protein
KW  - biomarker
Y1  - 2018
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/52074
SN  - 1424-8220
VL  - 18
IS  - 9
PB  - MDPI
CY  - Basel
ER  -