TY  - JOUR
A1  - Machens, Fabian
A1  - Balazadeh, Salma
A1  - Müller-Röber, Bernd
A1  - Messerschmidt, Katrin
T1  - Synthetic Promoters and Transcription Factors for Heterologous Protein Expression in Saccharomyces cerevisiae
T2  - Frontiers in Bioengineering and Biotechnology
N2  - Orthogonal systems for heterologous protein expression as well as for the engineering of synthetic gene regulatory circuits in hosts like Saccharomyces cerevisiae depend on synthetic transcription factors (synTFs) and corresponding cis-regulatory binding sites. We have constructed and characterized a set of synTFs based on either transcription activator-like effectors or CRISPR/Cas9, and corresponding small synthetic promoters (synPs) with minimal sequence identity to the host’s endogenous promoters. The resulting collection of functional synTF/synP pairs confers very low background expression under uninduced conditions, while expression output upon induction of the various synTFs covers a wide range and reaches induction factors of up to 400. The broad spectrum of expression strengths that is achieved will be useful for various experimental setups, e.g., the transcriptional balancing of expression levels within heterologous pathways or the construction of artificial regulatory networks. Furthermore, our analyses reveal simple rules that enable the tuning of synTF expression output, thereby allowing easy modification of a given synTF/synP pair. This will make it easier for researchers to construct tailored transcriptional control systems.
KW  - JUB1
KW  - synthetic biology
KW  - transcriptional regulation
KW  - gene expression
KW  - synthetic circuits
KW  - dead Cas9
KW  - chimeric transcription factors
Y1  - 2017
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/40379
SN  - 2296-4185
VL  - 5
SP  - 1
EP  - 11
PB  - Frontiers
CY  - Lausanne
ER  -