TY  - JOUR
A1  - Schmieder, Peter
A1  - Nitschke, Felix
A1  - Steup, Martin
A1  - Mallow, Keven
A1  - Specker, Edgar
T1  - Determination of glucan phosphorylation using heteronuclear H-1,C-13 double and H-1,C-13,P-31 triple-resonance NMR spectra
T2  - Magnetic resonance in chemistry
N2  - Phosphorylation and dephosphorylation of starch and glycogen are important for their physicochemical properties and also their physiological functions. It is therefore desirable to reliably determine the phosphorylation sites. Heteronuclear multidimensional NMR-spectroscopy is in principle a straightforward analytical approach even for complex carbohydrate molecules. With heterogeneous samples from natural sources, however, the task becomes more difficult because a full assignment of the resonances of the carbohydrates is impossible to obtain. Here, we show that the combination of heteronuclear H-1,C-13 and H-1,C-13,P-31 techniques and information derived from spectra of a set of reference compounds can lead to an unambiguous determination of the phosphorylation sites even in heterogeneous samples.
KW  - heteronuclear NMR
KW  - triple resonance
KW  - phosphorylation
KW  - starch
Y1  - 2013
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/34718
SN  - 0749-1581
VL  - 51
IS  - 10
SP  - 655
EP  - 661
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -