TY - JOUR A1 - Steffen, Jenny A1 - von Nickisch-Rosenegk, Markus A1 - Bier, Frank Fabian T1 - In vitro transcription of a whole gene on a surface-coupled template N2 - An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated Y1 - 2005 UR - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/13889 SN - 1473-0189 ER -