TY  - JOUR
A1  - Holzlöhner, Pamela
A1  - Butze, Monique
A1  - Maier, Natalia
A1  - Hebel, Nicole
A1  - Schliebs, Erik
A1  - Micheel, Burkhard
A1  - Fuener, Jonas
A1  - Heidicke, Gabriele
A1  - Hanack, Katja
T1  - Generation of murine monoclonal antibodies with specificity against conventional camelid IgG1 and heavy-chain only IgG2/3
T2  - Veterinary Immunology and Immunopathology
N2  - Camelids possess antibodies with a conventional four-chain structure consisting of two heavy and two light chains (of subclass IgG1) but further they also generate heavy-chain only antibodies (of subclass IgG2 and 3) which are fully functional in antigen binding. In this study subclass-specific murine monoclonal antibodies specific to conventional camelid IgG1 and heavy-chain only IgG2/3 were generated and validated for the use as potent secondary detection reagents. The monoclonal antibodies are able to differentiate between all camelid IgGs, conventional four-chain camelid antibodies (of subclass IgG1) and exclusively heavy chain-only antibodies (of subclasses IgG2 and IgG3). Further these antibodies were used to detect specific immune responses after vaccination of Camelids against bovine corona- and rotavirus strains and different E.coli. and Clostridia - antigens and to identify Erysipelothrix rhusiopathiae infected animals within a herd. The described antibodies are suitable as new secondary agents for the detection of different camelid subclasses and the validation of camelid immune reactions.
KW  - Camelid antibodies
KW  - Heavy-chain only antibodies
KW  - Monoclonal antibodies
KW  - Secondary antibodies
KW  - Vaccination
KW  - Disease monitoring
Y1  - 2018
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/53444
SN  - 0165-2427
SN  - 1873-2534
VL  - 197
SP  - 1
EP  - 6
PB  - Elsevier
CY  - Amsterdam
ER  -