Gene structures and processing of Arabidopsis thaliana HYL1-dependent pri-miRNAs

  • Arabidopsis thaliana HYL1 is a nuclear doublestranded RNA-binding protein involved in the maturation of pri-miRNAs. A quantitative real-time PCR platform for parallel quantification of 176 primiRNAs was used to reveal strong accumulation of 57 miRNA precursors in the hyl1 mutant that completely lacks HYL1 protein. This approach enabled us for the first time to pinpoint particular members of MIRNA family genes that require HYL1 activity for efficient maturation of their precursors. Moreover, the accumulation of miRNA precursors in the hyl1 mutant gave us the opportunity to carry out 3’ and 5’ RACE experiments which revealed that some of these precursors are of unexpected length. The alignment of HYL1- dependent miRNA precursors to A. thaliana genomic sequences indicated the presence of introns in 12 out of 20 genes studied. Some of the characterized intron-containing pri-miRNAs undergo alternative splicing such as exon skipping or usage of alternative 5’ splice sites suggesting that this process plays a role in the regulation of miRNA Arabidopsis thaliana HYL1 is a nuclear doublestranded RNA-binding protein involved in the maturation of pri-miRNAs. A quantitative real-time PCR platform for parallel quantification of 176 primiRNAs was used to reveal strong accumulation of 57 miRNA precursors in the hyl1 mutant that completely lacks HYL1 protein. This approach enabled us for the first time to pinpoint particular members of MIRNA family genes that require HYL1 activity for efficient maturation of their precursors. Moreover, the accumulation of miRNA precursors in the hyl1 mutant gave us the opportunity to carry out 3’ and 5’ RACE experiments which revealed that some of these precursors are of unexpected length. The alignment of HYL1- dependent miRNA precursors to A. thaliana genomic sequences indicated the presence of introns in 12 out of 20 genes studied. Some of the characterized intron-containing pri-miRNAs undergo alternative splicing such as exon skipping or usage of alternative 5’ splice sites suggesting that this process plays a role in the regulation of miRNA biogenesis. In the hyl1 mutant intron-containing pri-miRNAs accumulate alongside spliced primiRNAs suggesting the recruitment of HYL1 into the miRNA precursor maturation pathway before their splicing occurs.show moreshow less

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Author:Bogna Szarzynska, Lukasz Sobkowiak, Bikram Datt Pant, Salma Balazadeh, Wolf-Rüdiger Scheible, Bernd Müller-Röber, Artur Jarmolowski, Zofia Szweykowska-Kulinska
URN:urn:nbn:de:kobv:517-opus-45085
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe, ISSN 1866-8372 (paper 138)
Document Type:Postprint
Language:English
Date of Publication (online):2010/07/19
Year of Completion:2009
Publishing Institution:Universität Potsdam
Release Date:2010/07/19
Tag:Abscisic-acid; Binding-protein hyl1; Flowering time; Micro-RNA; Serrate
Source:Nucleic acids research 37 (2009), 9, S. 3083 - 3093, DOI: 10.1093/nar/gkp189
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung, Nicht kommerziell, Weitergabe zu gleichen Bedingungen 2.0 Deutschland
Notes extern:
The article was originally published by Oxford University Press:
Nucleic Acids Research. - 37 (2009), 9, S. 3083-3093
ISSN 0305-1048
DOI 10.1093/nar/gkp189