Analysis of two D1-like dopamine receptors from the honey bee Apis mellifera reveals agonist-independent activity

  • Dopamine is found in many invertebrate organisms, including insects, however, the mechanisms through which this amine operates remain unclear. We have expressed two dopamine receptors cloned from honey bee (AmDOP1 and AmDOP2) in insect cells (Spodoptera frugiperda), and compared their pharmacology directly using production of cAMP as a functional assay. In each assay, AmDOP1 receptors required lower concentrations of dopamine and 6,7-ADTN for maximal activation than AmDOP2 receptors. Conversely, butaclamol and cis(Z)-flupentixol were more potent at blocking the cAMP response mediated through AmDOP2 than AmDOP1 receptors. Expression of AmDOP1, but not AmDOP2, receptors significantly increased levels of cAMP even in the absence of ligand. This constitutive activity was blocked by cis(Z)-flupentixol. This work provides the first evidence of a constitutively activated dopamine receptor in invertebrates and suggests that although AmDOP1 and AmDOP2 share much less homology than their vertebrate counterparts, they display a number of functioDopamine is found in many invertebrate organisms, including insects, however, the mechanisms through which this amine operates remain unclear. We have expressed two dopamine receptors cloned from honey bee (AmDOP1 and AmDOP2) in insect cells (Spodoptera frugiperda), and compared their pharmacology directly using production of cAMP as a functional assay. In each assay, AmDOP1 receptors required lower concentrations of dopamine and 6,7-ADTN for maximal activation than AmDOP2 receptors. Conversely, butaclamol and cis(Z)-flupentixol were more potent at blocking the cAMP response mediated through AmDOP2 than AmDOP1 receptors. Expression of AmDOP1, but not AmDOP2, receptors significantly increased levels of cAMP even in the absence of ligand. This constitutive activity was blocked by cis(Z)-flupentixol. This work provides the first evidence of a constitutively activated dopamine receptor in invertebrates and suggests that although AmDOP1 and AmDOP2 share much less homology than their vertebrate counterparts, they display a number of functional parallels with the mammalian D1-like dopamine receptors.show moreshow less

Download full text files

Export metadata

  • Export Bibtex
  • Export RIS
  • Export XML

Additional Services

Share in Twitter Search Google Scholar
Metadaten
Author:Wolfgang Blenau, Julie A. Mustard, Ingrid S. Hamilton, Vernon K. Ward, Paul R. Ebert, Alison R. Mercer
URN:urn:nbn:de:kobv:517-opus-44378
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe, ISSN 1866-8372 (paper 109)
Document Type:Postprint
Language:English
Date of Publication (online):2010/07/02
Year of Completion:2003
Publishing Institution:Universität Potsdam
Release Date:2010/07/02
Tag:Baculovirus; Biogenic amine; G protein-coupled receptor; Invertebrate; cAMP
Source:Brain research : Molecular brain research 113 (2003), 1-2, S. 67 - 77, DOI 10.1016/S0169-328X(03)00091-3
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoKeine Nutzungslizenz vergeben - es gilt das deutsche Urheberrecht
Notes extern:
The article was originally published by:
Elsevier
Brain research : Molecular brain research. - 113 (2003), 1-2, S. 67-77
ISSN 0169-328X
DOI 10.1016/S0169-328X(03)00091-3