An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants

  • A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen-sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong autofluorescence of the plant tissue. The method was established using photosynthesis-competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species.

Download full text files

Export metadata

  • Export Bibtex
  • Export RIS
  • Export XML

Additional Services

Share in Twitter Search Google Scholar
Metadaten
Author:Elmar Schmälzlin, Joost T. van Dongen, Ingo Klimant, Bettina Marmodée, Martin Steup, Joachim Fishahn, Peter Geigenberger, Hans-Gerd Löhmannsröben
URN:urn:nbn:de:kobv:517-opus-12232
Series (Serial Number):Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe, ISSN 1866-8372 (paper 007)
Document Type:Postprint
Language:English
Date of Publication (online):2007/01/26
Year of Completion:2005
Publishing Institution:Universität Potsdam
Release Date:2007/01/26
Source:Biophysical Journal. - ISSN 1542-0086 (online). - 89 (2005), S. 1339 - 1345
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Collections:Universität Potsdam / Aufsätze (Pre- und Postprints) / Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie / Physikalische Chemie
Notes extern:
first published in:
Biophysical Journal. - ISSN 1542-0086. - 89 (2005), S. 1339 - 1345
doi: 10.1529/biophysj.105.063453