TY  - JOUR
A1  - Hildebrandt, Niko
A1  - Charbonniere, Loïc J.
A1  - Beck, Michael
A1  - Ziessel, Raymond F.
A1  - Löhmannsröben, Hans-Gerd
T1  - Quantum dots as efficient energy acceptors in a time-resolved fluoroimmunoassay
Y1  - 2005
SN  - 1433-7851
ER  - 
TY  - GEN
A1  - Hildebrandt, Nico
A1  - Charbonnière, Loïc J.
A1  - Ziessel, Raymond F.
A1  - Löhmannsröben, Hans-Gerd
T1  - Quantum dots as resonance energy transfer acceptors for monitoring biological interactions
N2  - Quantum dots (QDs) are common as luminescing markers for imaging in biological applications because their optical properties seem to be inert against their surrounding solvent. This, together with broad and strong absorption bands and intense, sharp tuneable luminescence bands, makes them interesting candidates for methods utilizing Forster Resonance Energy Transfer (FRET), e. g. for sensitive homogeneous fluoroimmunoassays (FIA). In this work we demonstrate energy transfer from Eu3+-trisbipyridin (Eu-TBP) donors to CdSe-ZnS-QD acceptors in solutions with and without serum. The QDs are commercially available CdSe-ZnS core-shell particles emitting at 655 nm (QD655). The FRET system was achieved by the binding of the streptavidin conjugated donors with the biotin conjugated acceptors. After excitation of Eu-TBP and as result of the energy transfer, the luminescence of the QD655 acceptors also showed lengthened decay times like the donors. The energy transfer efficiency, as calculated from the decay times of the bound and the unbound components, amounted to 37%. The Forster-radius, estimated from the absorption and emission bands, was ca. 77Å. The effective binding ratio, which not only depends on the ratio of binding pairs but also on unspecific binding, was obtained from the donor emission dependent on the concentration. As serum promotes unspecific binding, the overall FRET efficiency of the assay was reduced. We conclude that QDs are good substitutes for acceptors in FRET if combined with slow decay donors like Europium. The investigation of the influence of the serum provides guidance towards improving binding properties of QD assays.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 012 
Y1  - 2006
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-12213
ER  - 
TY  - JOUR
A1  - Geißler, Daniel
A1  - Charbonnière, Loïc J.
A1  - Ziessel, Raymond F.
A1  - Butlin, Nathaniel G.
A1  - Löhmannsröben, Hans-Gerd
A1  - Hildebrandt, Niko
T1  - Quantum dot biosensors for ultrasensitive multiplexed diagnostics
N2  - Time- and color-resolved detection of Foerster resonance energy transfer (FRET) from luminescent terbium complexes to different semiconductor quantum dots results in a fivefold multiplexed bioassay with sub-picomolar detection limits for all five bioanalytes (see picture). The detection of up to five biomarkers occurs with a sensitivity that is 40-240-fold higher than one of the best-established single-analyte reference assays.
Y1  - 2010
UR  - http://www3.interscience.wiley.com/cgi-bin/jhome/26737/
U6  - https://doi.org/10.1002/anie.200906399
SN  - 1433-7851
ER  - 
TY  - JOUR
A1  - Geißler, Daniel
A1  - Charbonnière, Loïc J.
A1  - Ziessel, Raymond F.
A1  - Löhmannsröben, Hans-Gerd
T1  - Quantum dots as FRET acceptors for highly sensitive multiplexing immunoassays
Y1  - 2009
ER  -