TY  - JOUR
A1  - Song, Yu
A1  - Li, Gang
A1  - Nowak, Jacqueline
A1  - Zhang, Xiaoqing
A1  - Xu, Dongbei
A1  - Yang, Xiujuan
A1  - Huang, Guoqiang
A1  - Liang, Wanqi
A1  - Yang, Litao
A1  - Wang, Canhua
A1  - Bulone, Vincent
A1  - Nikoloski, Zoran
A1  - Hu, Jianping
A1  - Persson, Staffan
A1  - Zhang, Dabing
T1  - The Rice Actin-Binding Protein RMD Regulates Light-Dependent Shoot Gravitropism
JF  - Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants
N2  - Light and gravity are two key determinants in orientating plant stems for proper growth and development. The organization and dynamics of the actin cytoskeleton are essential for cell biology and critically regulated by actin-binding proteins. However, the role of actin cytoskeleton in shoot negative gravitropism remains controversial. In this work, we report that the actin-binding protein Rice Morphology Determinant (RMD) promotes reorganization of the actin cytoskeleton in rice (Oryza sativa) shoots. The changes in actin organization are associated with the ability of the rice shoots to respond to negative gravitropism. Here, light-grown rmd mutant shoots exhibited agravitropic phenotypes. By contrast, etiolated rmd shoots displayed normal negative shoot gravitropism. Furthermore, we show that RMD maintains an actin configuration that promotes statolith mobility in gravisensing endodermal cells, and for proper auxin distribution in light-grown, but not dark-grown, shoots. RMD gene expression is diurnally controlled and directly repressed by the phytochrome-interacting factor-like protein OsPIL16. Consequently, overexpression of OsPIL16 led to gravisensing and actin patterning defects that phenocopied the rmd mutant. Our findings outline a mechanism that links light signaling and gravity perception for straight shoot growth in rice.
Y1  - 2019
U6  - https://doi.org/10.1104/pp.19.00497
SN  - 0032-0889
SN  - 1532-2548
VL  - 181
IS  - 2
SP  - 630
EP  - 644
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  - 
TY  - JOUR
A1  - Yu, Yanjun
A1  - Wu, Shenjie
A1  - Nowak, Jacqueline
A1  - Wang, Guangda
A1  - Han, Libo
A1  - Feng, Zhidi
A1  - Mendrinna, Amelie
A1  - Ma, Yinping
A1  - Wang, Huan
A1  - Zhang, Xiaxia
A1  - Tian, Juan
A1  - Dong, Li
A1  - Nikoloski, Zoran
A1  - Persson, Staffan
A1  - Kong, Zhaosheng
T1  - Live-cell imaging of the cytoskeleton in elongating cotton fibres
JF  - Nature plants
N2  - Cotton (Gossypium hirsutum) fibres consist of single cells that grow in a highly polarized manner, assumed to be controlled by the cytoskeleton(1-3). However, how the cytoskeletal organization and dynamics underpin fibre development remains unexplored. Moreover, it is unclear whether cotton fibres expand via tip growth or diffuse growth(2-4). We generated stable transgenic cotton plants expressing fluorescent markers of the actin and microtubule cytoskeleton. Live-cell imaging revealed that elongating cotton fibres assemble a cortical filamentous actin network that extends along the cell axis to finally form actin strands with closed loops in the tapered fibre tip. Analyses of F-actin network properties indicate that cotton fibres have a unique actin organization that blends features of both diffuse and tip growth modes. Interestingly, typical actin organization and endosomal vesicle aggregation found in tip-growing cell apices were not observed in fibre tips. Instead, endomembrane compartments were evenly distributed along the elongating fibre cells and moved bi-directionally along the fibre shank to the fibre tip. Moreover, plus-end tracked microtubules transversely encircled elongating fibre shanks, reminiscent of diffusely growing cells. Collectively, our findings indicate that cotton fibres elongate via a unique tip-biased diffuse growth mode.
Y1  - 2019
U6  - https://doi.org/10.1038/s41477-019-0418-8
SN  - 2055-026X
SN  - 2055-0278
VL  - 5
IS  - 5
SP  - 498
EP  - 504
PB  - Nature Publ. Group
CY  - London
ER  -