TY  - GEN
A1  - Szarzynska, Bogna
A1  - Sobkowiak, Lukasz
A1  - Pant, Bikram Datt
A1  - Balazadeh, Salma
A1  - Scheible, Wolf-Rüdiger
A1  - Müller-Röber, Bernd
A1  - Jarmolowski, Artur
A1  - Szweykowska-Kulinska, Zofia
T1  - Gene structures and processing of Arabidopsis thaliana HYL1-dependent pri-miRNAs
N2  - Arabidopsis thaliana HYL1 is a nuclear doublestranded RNA-binding protein involved in the maturation of pri-miRNAs. A quantitative real-time PCR platform for parallel quantification of 176 primiRNAs was used to reveal strong accumulation of 57 miRNA precursors in the hyl1 mutant that completely lacks HYL1 protein. This approach enabled us for the first time to pinpoint particular members of MIRNA family genes that require HYL1 activity for efficient maturation of their precursors. Moreover, the accumulation of miRNA precursors in the hyl1 mutant gave us the opportunity to carry out 3’ and 5’ RACE experiments which revealed that some of these precursors are of unexpected length. The alignment of HYL1- dependent miRNA precursors to A. thaliana genomic sequences indicated the presence of introns in 12 out of 20 genes studied. Some of the characterized intron-containing pri-miRNAs undergo alternative splicing such as exon skipping or usage of alternative 5’ splice sites suggesting that this process plays a role in the regulation of miRNA biogenesis. In the hyl1 mutant intron-containing pri-miRNAs accumulate alongside spliced primiRNAs suggesting the recruitment of HYL1 into the miRNA precursor maturation pathway before their splicing occurs.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - paper 138 
KW  - Binding-protein hyl1
KW  - Abscisic-acid
KW  - Flowering time
KW  - Micro-RNA
KW  - Serrate
Y1  - 2010
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/4312
UR  - https://nbn-resolving.org/urn:nbn:de:kobv:517-opus-45085
ER  -