Martin Reichel, Cosima Rhein, Lena M. Hofmann, Juliana Monti, Lukasz Japtok, Dominik Langgartner, Andrea M. Füchsl, Burkhard Kleuser, Erich Gulbins, Claus Hellerbrand, Stefan O. Reber, Johannes Kornhuber
- Chronic psychosocial stress adversely affects human morbidity and is a risk factor for inflammatory disorders, liver diseases, obesity, metabolic syndrome, and major depressive disorder (MDD). In recent studies, we found an association of MDD with an increase of acid sphingomyelinase (ASM) activity. Thus, we asked whether chronic psychosocial stress as a detrimental factor contributing to the emergence of MDD would also affect ASM activity and sphingolipid (SL) metabolism. To induce chronic psychosocial stress in male mice we employed the chronic subordinate colony housing (CSC) paradigm and compared them to non-stressed single housed control (SHC) mice. We determined Asm activity in liver and serum, hepatic SL concentrations as well as hepatic mRNA expression of genes involved in SL metabolism. We found that hepatic Asm activity was increased by 28% (P = 0.006) and secretory Asm activity by 47% (P = 0.002) in stressed mice. C16:0-Cer was increased by 40% (P = 0.008). Gene expression analysis further revealed an increased expressionChronic psychosocial stress adversely affects human morbidity and is a risk factor for inflammatory disorders, liver diseases, obesity, metabolic syndrome, and major depressive disorder (MDD). In recent studies, we found an association of MDD with an increase of acid sphingomyelinase (ASM) activity. Thus, we asked whether chronic psychosocial stress as a detrimental factor contributing to the emergence of MDD would also affect ASM activity and sphingolipid (SL) metabolism. To induce chronic psychosocial stress in male mice we employed the chronic subordinate colony housing (CSC) paradigm and compared them to non-stressed single housed control (SHC) mice. We determined Asm activity in liver and serum, hepatic SL concentrations as well as hepatic mRNA expression of genes involved in SL metabolism. We found that hepatic Asm activity was increased by 28% (P = 0.006) and secretory Asm activity by 47% (P = 0.002) in stressed mice. C16:0-Cer was increased by 40% (P = 0.008). Gene expression analysis further revealed an increased expression of tumor necrosis factor (TNF)-alpha (P = 0.009) and of several genes involved in SL metabolism (Cers5, P = 0.028; Cers6, P = 0.045; Gba, P = 0.049; Gba2, P = 0.030; Ormdl2, P = 0.034; Smpdl3B; P = 0.013). Our data thus provides first evidence that chronic psychosocial stress, at least in mice, induces alterations in SL metabolism, which in turn might be involved in mediating the adverse health effects of chronic psychosocial stress and peripheral changes occurring in mood disorders.…
MetadatenAuthor details: | Martin ReichelORCiD, Cosima RheinORCiD, Lena M. Hofmann, Juliana Monti, Lukasz JaptokGND, Dominik Langgartner, Andrea M. Füchsl, Burkhard KleuserORCiDGND, Erich GulbinsORCiDGND, Claus Hellerbrand, Stefan O. Reber, Johannes KornhuberORCiD |
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DOI: | https://doi.org/10.3389/fpsyt.2018.00496 |
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ISSN: | 1664-0640 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30386262 |
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Title of parent work (English): | Frontiers in Psychiatry |
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Publisher: | Frontiers Research Foundation |
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Place of publishing: | Lausanne |
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Publication type: | Article |
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Language: | English |
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Year of first publication: | 2018 |
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Publication year: | 2018 |
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Release date: | 2021/09/17 |
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Tag: | acid sphingomyelinase; ceramide; chronic psychosocial stress; chronic subordinate colony housing (CSC); liver metabolism; sphingolipid metabolism |
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Volume: | 9 |
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Number of pages: | 8 |
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Funding institution: | DFGGerman Research Foundation (DFG) [GU 335/29-1, KO 947/13-1]; BMBFFederal Ministry of Education & Research (BMBF) [01EE1401G, 01EE1401C] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
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DDC classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Peer review: | Referiert |
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Publishing method: | Open Access / Gold Open-Access |
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| DOAJ gelistet |
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External remark: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1120 |
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